Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2010-07-02 |
タイトル |
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タイトル |
[原著]An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody |
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言語 |
ja |
作成者 |
Nakasone,Toshiyuki
Hanashiro, Kazuhiko
Nakamura, Mariko
Sunakawa, Hajime
Kosugi, Tadayoshi
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
ja |
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権利情報 |
琉球医学会 |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
monoclonal antibody against rat IgE |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
rat IgE |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
DNP-specific rat IgE |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
IgE-capture ELISA |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
A hybridoma producing monoclonal antibody against monoclonal DNP-specific rat IgE (IgE FE-3) was generated by fusion of P3-X63-Ag8-Ul (P3U1) mouse myeloma and spleen cells from BALB/c mice immunized with IgE FE-3. Five hybridoma clones were obtained. Three clones of them had a high reactivity against IgE FE-3 and a low reactivity against the L-chain of IgE FE-3 on ELISA analysis for the first screening of the hybridoma. The binding of monoclonal antibodies produced from clone BB-9 to IgE FE-3 revealed the strongest inhibition among three monoclonal antibodies on pretreatment with polyclonal rabbit anti-IgE FE-3 antibodies. It was clarified that monoclonal antibody BB-9 reacted specifically with the heavy chain of IgE FE-3 by Western blotting analysis. Furthermore, the reactivity of the monoclonal antibody BB-9 to IgE FE-3 was higher than that of MARE-1, which is a monoclonal antibody against rat myeloma IgE. The monoclonal antibody BB-9 was therefore employed for IgE-capture ELISA to determine the concentration of IgE FE-3. No significant alterations of color development occurred when igE FE-3 was concomitantly incubated with large excesses of IgG antibodies and rat myeloma IgE. Our data indicated that IgE-capture ELISA employing the monoclonal antibody BB-9 had a higher sensitivity for the determination of IgE FE-3 than did that using polyclonal rabbit anti-IgE FE-3 antibodies. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
ja |
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出版者 |
琉球医学会 |
出版者 |
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言語 |
en |
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出版者 |
Ryukyu Medical Association |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346888X |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0289-1530 |
収録物識別子 |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN10369445 |
収録物名 |
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言語 |
ja |
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収録物名 |
琉球医学会誌 = Ryukyu Medical Journal |
書誌情報 |
巻 20,
号 3,
p. 129-135,
発行日 2001
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