{"_buckets": {"deposit": "b9cf018c-e3e0-45ee-87f4-01f8dcad7674"}, "_deposit": {"id": "2016140", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2016140"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02016140", "sets": ["1642838216134", "1642838412624"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "[原著]An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakasone,Toshiyuki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Hanashiro, Kazuhiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nakamura, Mariko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Sunakawa, Hajime", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kosugi, Tadayoshi", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "ja", "subitem_1522651041219": "琉球医学会"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "monoclonal antibody against rat IgE"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "rat IgE"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "DNP-specific rat IgE"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "IgE-capture ELISA"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "A hybridoma producing monoclonal antibody against monoclonal DNP-specific rat IgE (IgE FE-3) was generated by fusion of P3-X63-Ag8-Ul (P3U1) mouse myeloma and spleen cells from BALB/c mice immunized with IgE FE-3. Five hybridoma clones were obtained. Three clones of them had a high reactivity against IgE FE-3 and a low reactivity against the L-chain of IgE FE-3 on ELISA analysis for the first screening of the hybridoma. The binding of monoclonal antibodies produced from clone BB-9 to IgE FE-3 revealed the strongest inhibition among three monoclonal antibodies on pretreatment with polyclonal rabbit anti-IgE FE-3 antibodies. It was clarified that monoclonal antibody BB-9 reacted specifically with the heavy chain of IgE FE-3 by Western blotting analysis. Furthermore, the reactivity of the monoclonal antibody BB-9 to IgE FE-3 was higher than that of MARE-1, which is a monoclonal antibody against rat myeloma IgE. The monoclonal antibody BB-9 was therefore employed for IgE-capture ELISA to determine the concentration of IgE FE-3. No significant alterations of color development occurred when igE FE-3 was concomitantly incubated with large excesses of IgG antibodies and rat myeloma IgE. Our data indicated that IgE-capture ELISA employing the monoclonal antibody BB-9 had a higher sensitivity for the determination of IgE FE-3 than did that using polyclonal rabbit anti-IgE FE-3 antibodies.", "subitem_description_type": "Other"}, {"subitem_description": "論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球医学会"}, {"subitem_1522300295150": "en", "subitem_1522300316516": "Ryukyu Medical Association"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "1346888X"}, {"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0289-1530"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN10369445"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球医学会誌 = Ryukyu Medical Journal"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2001", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "135", "bibliographicPageStart": "129", "bibliographicVolumeNumber": "20"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "v20p129.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2016140/files/v20p129.pdf"}, "version_id": "f39243dd-994c-4a63-878b-1873c26bc324"}]}, "item_title": "[原著]An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody", "item_type_id": "15", "owner": "1", "path": ["1642838216134", "1642838412624"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002016140", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2010-07-02"}, "publish_date": "2010-07-02", "publish_status": "0", "recid": "2016140", "relation": {}, "relation_version_is_last": true, "title": ["[原著]An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody"], "weko_shared_id": -1}
[原著]An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody
http://hdl.handle.net/20.500.12000/0002016140
http://hdl.handle.net/20.500.12000/0002016140