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[原著]A preliminary study on the effect of myeloperoxidase on etoposide-induced HPRT mutants in HL-60 cells
http://hdl.handle.net/20.500.12000/0002016162
http://hdl.handle.net/20.500.12000/0002016162a4a3acde-a14e-4fdf-91ed-fb99bceaef33
名前 / ファイル | ライセンス | アクション |
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v23p85.pdf
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Item type | デフォルトアイテムタイプ(フル)(1) | |||||||
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公開日 | 2010-07-02 | |||||||
タイトル | ||||||||
タイトル | [原著]A preliminary study on the effect of myeloperoxidase on etoposide-induced HPRT mutants in HL-60 cells | |||||||
言語 | ja | |||||||
作成者 |
Zheng, Kui-Cheng
× Zheng, Kui-Cheng
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アクセス権 | ||||||||
アクセス権 | open access | |||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
権利情報 | ||||||||
言語 | ja | |||||||
権利情報 | 琉球医学会 | |||||||
主題 | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | etoposide | |||||||
主題 | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | myeloperoxidase | |||||||
主題 | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | hypoxanthine phosphoribosyl transferase gene | |||||||
主題 | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | HL-60 cells | |||||||
主題 | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | mutants | |||||||
内容記述 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | Topoisomerase II (Topo II) -directed antitumor drug, etoposide (VP-16) -related leukemia (T-cell acute myelogenous leukemia, t-AML) occurs in about 10% of drug-treated patients. Understanding the mechanisms underlying leukemia induced by VP-16 is important for the development of safer drugs against leukemia. It has been postulated that redox cycling of VP-16 initiated by myeloperoxidase (MPO) produces reactive oxygen species responsible for the genotoxicity of this agent in myeloid precursors, leading to chromosomal translocations responsible for t-AML. To test this hypothesis, MPO activity of HL-60 cells were down-regulated by treating the cells with succinyl acetone (SA). The genotoxicity of VP-16 in these cells was then determined by analyzing the frequencies of mutations in the hypoxanthine phosphoribosyl transferase (HPRT) gene and compared with that in cells without prior SA-treatment. The results showed that the VP-16-induced HPRT mutant frequency in cells whose MPO activity was inhibited by prior SA treatment was significantly decreased by 78.8%, as compared with cells without prior SA-treatment. These results support our hypothesis that VP-16 induces genotoxicity and carcinogenicity through MPOmediated oxidative activation of VP-16. | |||||||
内容記述 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | 論文 | |||||||
出版者 | ||||||||
言語 | ja | |||||||
出版者 | 琉球医学会 | |||||||
出版者 | ||||||||
言語 | en | |||||||
出版者 | Ryukyu Medical Association | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源タイプ | journal article | |||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
出版タイプ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
収録物識別子 | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 1346888X | |||||||
収録物識別子 | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 0289-1530 | |||||||
収録物識別子 | ||||||||
収録物識別子タイプ | NCID | |||||||
収録物識別子 | AN10369445 | |||||||
収録物名 | ||||||||
言語 | ja | |||||||
収録物名 | 琉球医学会誌 = Ryukyu Medical Journal | |||||||
書誌情報 |
巻 23, 号 3, p. 85-91 |