Item type |
デフォルトアイテムタイプ(フル)(1) |
公開日 |
2010-07-02 |
タイトル |
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タイトル |
[原著]Bile endothelin-1 as a tool for diagnosig ischemia-reperfusion injury |
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言語 |
ja |
作成者 |
Miyaguni, Takao
Shiraishi, Masayuki
Tomori, Hirofumi
Okuhama, Yukihiro
Higa, Takao
Muto, Yoshihiro
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
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言語 |
ja |
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権利情報 |
琉球医学会 |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
ischemia-reperfusion injury |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
endothelin-1 |
主題 |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
liver |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
We investigated whether the extent of graft ischemia reperfusion (I/R) injury can be evaluated by assessing the level of bile and serum endothelin-1 (ET-1). In groups 1 to 3 (n=5 in each group), the livers of male Wistar rats were surgically isolated from the surrounding attachments and then perfused in-situ with heparinized cold saline (4℃) under a port-systemic shunt. The livers were then reperfused after a pre-determined cold ischemia time (0, 30, and 60 min in groups 1, 2, and 3, respectively). The ET-1 levels in either the bile or the serum obtained from the suprahepatic vena cava (SHVC) and abdominal aorta, were assessed from -1 hour to +72 hours after reperfusion in each group. In group 3, the ET-1 level at the SHVC increased to $19.9 \pm 2.88$ and $19.43 \pm 3.14$ pg/ml at 1.5 and 2 hr after reperfusion, respectively, which were significantly higher than those of group 1 ($13.6 \pm 2.73$, $13.05 0.58\pm $, p<0.05) and 2 ($11.67 \pm 2.21$, $12.2 \pm 2.22$, p<0.05). The bile ET-1 concentration in group 3 decreased at 1 hr after reperfusion ($14.22 \pm 6.26$ pg/ml), but increased in group 1 ($108.1 \pm 49.3$). The bile ET-1 concentration did not recover to its pre-ischemic level until 72 hr after reperfusion in group 3, but showed no apparent decrease throughout the experiment in groups 1 and 2. In immunohistochemistry, ET-1 expression on the central and portal vein was stronger in group 3 than in group 1, whereas the expression on the bile duct was weaker in group 3 than that in group 1. In conclusion, ET-1 was directly released from the damaged endothelial cells into the SHVC depending on the extent of the I/R injury, whereas the ET-1 excretion into the bile was inversely suppressed depending on the amount of I/R injury. These changes of ET-1 excretion might thus be useful in diagnosing hepatic I/R injury. |
内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
論文 |
出版者 |
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言語 |
ja |
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出版者 |
琉球医学会 |
出版者 |
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言語 |
en |
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出版者 |
Ryukyu Medical Association |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1346888X |
収録物識別子 |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0289-1530 |
収録物識別子 |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN10369445 |
収録物名 |
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言語 |
ja |
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収録物名 |
琉球医学会誌 = Ryukyu Medical Journal |
書誌情報 |
巻 20,
号 1,
p. 1-6
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