{"_buckets": {"deposit": "528d16ab-9ea0-4a3a-8f5a-7a239e032bdb"}, "_deposit": {"id": "2016560", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2016560"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02016560", "sets": ["1642838259014", "1642838412624"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "[報文]カラシナのカルス由来プロトプラストからの不定芽再分化", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "[ORIGINALS]Shoot Regeneration from Protoplasts Derived from Callus of Brassica Juncea", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "徳元, 正和", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "TOKUMOTO, Masakazu", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "ja", "subitem_1522651041219": "南方資源利用技術研究会"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "力ラシナカルスから単離したプロトプラストを用いて,初期分裂に及ぼす基本培地濃度,窒素塩濃度の影響,ホルモン濃度の影響,さらに不定芽再分化に対する各種添加物の効果について検討した.カラシナプロトプラストは浸透圧調節剤のマンニトール0.475～0.5Mの範囲で安定であった｡プロトプラストの初期分裂に対して,BA1mｇ/ lとNAAO.5mg/lのホルモン組合せで効果が高く,分裂率は29.4%であった. また,修正MS培地(NH_4NO_3を(NH_4)_2S0_4に置換)の窒素源として(NH_4)_2SO_4は200mg/l, KN0_3は475mg/lの濃度でもっとも高い分裂率が得られ,それぞれ34.7%,36.2%であった.修正MS培地中の無機塩濃度(窒素源は除く)は1/2濃度が効果的であった.得られた最適条件の培地に,活性炭(0.035%),カザミノ酸(0.025%),コンデショニング培地(50%)を添加して,プロトプラスト培養をすると,活性炭以外はコロニーを経てカルスが形成された.特にコンデショニング培地ではコロニーおよびカルス形成効果が高く,得られたカルスを再分化培地に移植すると,コンデショニング培地で不定芽形成(形成率12%)が見られた.しかし,他の培地では不定芽は形成されず不定根のみ形成された.", "subitem_description_type": "Other"}, {"subitem_description": "論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "南方資源利用技術研究会"}, {"subitem_1522300295150": "en", "subitem_1522300316516": "The Society of Tropical Resources Technologists"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0912-9588"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN10236281"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "南方資源利用技術研究会誌 = Journal of the society tropical resources technologists"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "1", "bibliographicPageEnd": "6", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "10"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "Vol10No1p1.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2016560/files/Vol10No1p1.pdf"}, "version_id": "78593f00-3fb4-4e81-9fe7-3542ce190ad9"}]}, "item_title": "[報文]カラシナのカルス由来プロトプラストからの不定芽再分化", "item_type_id": "15", "owner": "1", "path": ["1642838259014", "1642838412624"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002016560", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-10-26"}, "publish_date": "2014-10-26", "publish_status": "0", "recid": "2016560", "relation": {}, "relation_version_is_last": true, "title": ["[報文]カラシナのカルス由来プロトプラストからの不定芽再分化"], "weko_shared_id": -1}