| Item type |
デフォルトアイテムタイプ（フル）(1) |
| 公開日 |
2015-05-08 |
| タイトル |
|
|
タイトル |
[原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2 |
|
言語 |
ja |
| 作成者 |
Ulziikhishig, Enkhbaatar
Bayarjargal, Maitsetseg
Nonaka, Kimiko
Oshiro, Minoru
Kariya, Ken-ichi
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利情報 |
|
|
言語 |
ja |
|
権利情報 |
琉球医学会 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cancer cell phenotype |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Rap2 |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
MAP4K |
| 主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
skin cancer |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Purpose: Traf2- and Nck-interacting kinase (TNIK) is one of the effector kinases of the Ras-like GTPase Rap2, and belongs to the STE20 group of mitogen-activated protein kinase kinase kinase kinases (MAP4Ks). The purpose of this study was to explore the roles of TNIK in the phenotype of cancer cells. Results: Derivatives of a cutaneous squamous cell carcinoma (SCC) cell line, Pam212, in which expression of hemagglutinin-tagged TNIK (HA-TNIK) can be induced by modulating the concentration of a tetracycline derivative in culture medium were established by using a retrovirus-mediated gene transduction system. Morphological analyses revealed that HA-TNIK regulated intercellular adhesion and epithelial cell shape. A strong induction of HA-TNIK disrupted intercellular adhesion and abolished the epithelial cell shape. Moderately induced HA-TNIK co-localized with E-cadherin, a principal component of intercellular adherens junctions (AJs), and facilitated cellular detachment during hepatocyte growth factor-induced cell scattering and wound-healing cell migration assays. Moderately induced HA-TNIK also retarded Ca^<2_+>-dependent re-assembly of AJs that had been pre-disassembled by transient depletion of Ca^<2_+> in culture medium. Conclusion: These results complement our preceding work using a proteomic approach (Kuninaka et al. "Proteomic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2" Ryukyu Med. J. 26: 135-145, 2007) on roles of TNIK in SCC cells. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
論文 |
| 出版者 |
|
|
出版者 |
琉球医学会 |
|
言語 |
ja |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1346-888X |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10369445 |
| 収録物名 |
|
|
収録物名 |
琉球医学会誌 = Ryukyu Medical Journal |
|
言語 |
ja |
| 書誌情報 |
巻 32,
号 3･4,
p. 89-94
|
v3234p89.pdf
