ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "3b20a6f6-0e84-4b38-8ef1-e929e84fbbc9"}, "_deposit": {"id": "2017767", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2017767"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02017767", "sets": ["1642838229091", "1642838412624"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "[\u539f\u8457]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ulziikhishig, Enkhbaatar", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Bayarjargal, Maitsetseg", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nonaka, Kimiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Oshiro, Minoru", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kariya, Ken-ichi", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "ja", "subitem_1522651041219": "\u7409\u7403\u533b\u5b66\u4f1a"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "cancer cell phenotype"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Rap2"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "MAP4K"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "skin cancer"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Purpose: Traf2- and Nck-interacting kinase (TNIK) is one of the effector kinases of the Ras-like GTPase Rap2, and belongs to the STE20 group of mitogen-activated protein kinase kinase kinase kinases (MAP4Ks). The purpose of this study was to explore the roles of TNIK in the phenotype of cancer cells. Results: Derivatives of a cutaneous squamous cell carcinoma (SCC) cell line, Pam212, in which expression of hemagglutinin-tagged TNIK (HA-TNIK) can be induced by modulating the concentration of a tetracycline derivative in culture medium were established by using a retrovirus-mediated gene transduction system. Morphological analyses revealed that HA-TNIK regulated intercellular adhesion and epithelial cell shape. A strong induction of HA-TNIK disrupted intercellular adhesion and abolished the epithelial cell shape. Moderately induced HA-TNIK co-localized with E-cadherin, a principal component of intercellular adherens junctions (AJs), and facilitated cellular detachment during hepatocyte growth factor-induced cell scattering and wound-healing cell migration assays. Moderately induced HA-TNIK also retarded Ca^\u003c2_+\u003e-dependent re-assembly of AJs that had been pre-disassembled by transient depletion of Ca^\u003c2_+\u003e in culture medium. Conclusion: These results complement our preceding work using a proteomic approach (Kuninaka et al. \"Proteomic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2\" Ryukyu Med. J. 26: 135-145, 2007) on roles of TNIK in SCC cells.", "subitem_description_type": "Other"}, {"subitem_description": "\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u533b\u5b66\u4f1a"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "1346-888X"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN10369445"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u533b\u5b66\u4f1a\u8a8c = Ryukyu Medical Journal"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "3\uff654", "bibliographicPageEnd": "94", "bibliographicPageStart": "89", "bibliographicVolumeNumber": "32"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "v3234p89.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2017767/files/v3234p89.pdf"}, "version_id": "fe0668c2-68bb-4d90-a4d6-5cc8c575aa90"}]}, "item_title": "[\u539f\u8457]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2", "item_type_id": "15", "owner": "1", "path": ["1642838229091", "1642838412624"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002017767", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2015-05-08"}, "publish_date": "2015-05-08", "publish_status": "0", "recid": "2017767", "relation": {}, "relation_version_is_last": true, "title": ["[\u539f\u8457]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2"], "weko_shared_id": -1}
  1. 学術雑誌論文
  2. 琉球医学会
  3. 琉球医学会誌
  4. 32巻3・4号
  1. 部局別インデックス
  2. その他

[原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2

http://hdl.handle.net/20.500.12000/0002017767
http://hdl.handle.net/20.500.12000/0002017767
415aa00c-9e2c-4c62-9f4a-91701019ffb7
名前 / ファイル ライセンス アクション
v3234p89.pdf v3234p89.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2015-05-08
タイトル
タイトル [原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2
言語 ja
作成者 Ulziikhishig, Enkhbaatar

× Ulziikhishig, Enkhbaatar

en Ulziikhishig, Enkhbaatar

Bayarjargal, Maitsetseg

× Bayarjargal, Maitsetseg

en Bayarjargal, Maitsetseg

Nonaka, Kimiko

× Nonaka, Kimiko

en Nonaka, Kimiko

Oshiro, Minoru

× Oshiro, Minoru

en Oshiro, Minoru

Kariya, Ken-ichi

× Kariya, Ken-ichi

en Kariya, Ken-ichi

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 ja
権利情報 琉球医学会
主題
言語 en
主題Scheme Other
主題 cancer cell phenotype
言語 en
主題Scheme Other
主題 Rap2
言語 en
主題Scheme Other
主題 MAP4K
言語 en
主題Scheme Other
主題 skin cancer
内容記述
内容記述タイプ Other
内容記述 Purpose: Traf2- and Nck-interacting kinase (TNIK) is one of the effector kinases of the Ras-like GTPase Rap2, and belongs to the STE20 group of mitogen-activated protein kinase kinase kinase kinases (MAP4Ks). The purpose of this study was to explore the roles of TNIK in the phenotype of cancer cells. Results: Derivatives of a cutaneous squamous cell carcinoma (SCC) cell line, Pam212, in which expression of hemagglutinin-tagged TNIK (HA-TNIK) can be induced by modulating the concentration of a tetracycline derivative in culture medium were established by using a retrovirus-mediated gene transduction system. Morphological analyses revealed that HA-TNIK regulated intercellular adhesion and epithelial cell shape. A strong induction of HA-TNIK disrupted intercellular adhesion and abolished the epithelial cell shape. Moderately induced HA-TNIK co-localized with E-cadherin, a principal component of intercellular adherens junctions (AJs), and facilitated cellular detachment during hepatocyte growth factor-induced cell scattering and wound-healing cell migration assays. Moderately induced HA-TNIK also retarded Ca^<2_+>-dependent re-assembly of AJs that had been pre-disassembled by transient depletion of Ca^<2_+> in culture medium. Conclusion: These results complement our preceding work using a proteomic approach (Kuninaka et al. "Proteomic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2" Ryukyu Med. J. 26: 135-145, 2007) on roles of TNIK in SCC cells.
内容記述タイプ Other
内容記述 論文
出版者
言語 ja
出版者 琉球医学会
言語
言語 eng
資源タイプ
資源タイプ journal article
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 1346-888X
収録物識別子タイプ NCID
収録物識別子 AN10369445
収録物名
言語 ja
収録物名 琉球医学会誌 = Ryukyu Medical Journal
書誌情報
巻 32, 号 3・4, p. 89-94
戻る
0
views
See details
Views

Versions

Ver.1 2022-02-24 07:48:11.976875
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON

確認


Powered by WEKO3


Powered by WEKO3