{"_buckets": {"deposit": "3b20a6f6-0e84-4b38-8ef1-e929e84fbbc9"}, "_deposit": {"id": "2017767", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2017767"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02017767", "sets": ["1642838229091", "1642838412624"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "[原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ulziikhishig, Enkhbaatar", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Bayarjargal, Maitsetseg", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nonaka, Kimiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Oshiro, Minoru", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kariya, Ken-ichi", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "ja", "subitem_1522651041219": "琉球医学会"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "cancer cell phenotype"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Rap2"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "MAP4K"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "skin cancer"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Purpose: Traf2- and Nck-interacting kinase (TNIK) is one of the effector kinases of the Ras-like GTPase Rap2, and belongs to the STE20 group of mitogen-activated protein kinase kinase kinase kinases (MAP4Ks). The purpose of this study was to explore the roles of TNIK in the phenotype of cancer cells. Results: Derivatives of a cutaneous squamous cell carcinoma (SCC) cell line, Pam212, in which expression of hemagglutinin-tagged TNIK (HA-TNIK) can be induced by modulating the concentration of a tetracycline derivative in culture medium were established by using a retrovirus-mediated gene transduction system. Morphological analyses revealed that HA-TNIK regulated intercellular adhesion and epithelial cell shape. A strong induction of HA-TNIK disrupted intercellular adhesion and abolished the epithelial cell shape. Moderately induced HA-TNIK co-localized with E-cadherin, a principal component of intercellular adherens junctions (AJs), and facilitated cellular detachment during hepatocyte growth factor-induced cell scattering and wound-healing cell migration assays. Moderately induced HA-TNIK also retarded Ca^\u003c2_+\u003e-dependent re-assembly of AJs that had been pre-disassembled by transient depletion of Ca^\u003c2_+\u003e in culture medium. Conclusion: These results complement our preceding work using a proteomic approach (Kuninaka et al. \"Proteomic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2\" Ryukyu Med. J. 26: 135-145, 2007) on roles of TNIK in SCC cells.", "subitem_description_type": "Other"}, {"subitem_description": "論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球医学会"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "1346-888X"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN10369445"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球医学会誌 = Ryukyu Medical Journal"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "3･4", "bibliographicPageEnd": "94", "bibliographicPageStart": "89", "bibliographicVolumeNumber": "32"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "v3234p89.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2017767/files/v3234p89.pdf"}, "version_id": "fe0668c2-68bb-4d90-a4d6-5cc8c575aa90"}]}, "item_title": "[原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2", "item_type_id": "15", "owner": "1", "path": ["1642838229091", "1642838412624"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002017767", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2015-05-08"}, "publish_date": "2015-05-08", "publish_status": "0", "recid": "2017767", "relation": {}, "relation_version_is_last": true, "title": ["[原著]Phenotypic changes in a squamous cell carcinoma cell line induced by an effector kinase of a small G protein Rap2"], "weko_shared_id": -1}