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  1. 学位論文
  2. 博士論文
  3. 医学研究科
  1. 部局別インデックス
  2. 医学研究科

成人 T 細胞白血病・リンパ腫における生検組織を用いた新規診断アルゴリズム : HTLV-1 に対する RNA in situ hybridization と定量 PCR の組み合わせ

http://hdl.handle.net/20.500.12000/0002018043
http://hdl.handle.net/20.500.12000/0002018043
bb2ce9b2-d6f2-4982-821d-70bd40e8c6ee
名前 / ファイル ライセンス アクション
iken547abstract.pdf iken547abstract.pdf (875 KB)
iken547review.pdf iken547review.pdf (803 KB)
iken547text.pdf iken547text.pdf (3.1 MB)
Item type 琉球大学リポジトリ登録用アイテムタイプ(1)
公開日 2022-06-16
タイトル
タイトル 成人 T 細胞白血病・リンパ腫における生検組織を用いた新規診断アルゴリズム : HTLV-1 に対する RNA in situ hybridization と定量 PCR の組み合わせ
言語 ja
作成者 髙鳥, 光徳

× 髙鳥, 光徳

ja 髙鳥, 光徳

en Takatori, Mitsuyoshi

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利情報
言語 en
権利情報 © The Author(s) 2020.
言語 en
権利情報Resource http://creativecommons.org/licenses/by/4.0/
権利情報 Creative Commons Attribution 4.0 International
内容記述
内容記述タイプ Abstract
内容記述 Histopathological distinction between adult T-cell leuketnia/lymphoma (ATLL) and other T-cell neoplasms is often challenging. The current gold standard for the accurate diagnosis of ATLL is the Southern blot hybridization (SBH) assay, which detects clonal integration of human T-cell leuketnia virus type I (HTLV-1) provirus. However, SBH cannot be petformed with small biopsy or formalin-fixed paraffin-embedded (FFPE) tissue samples because this assay requires a large amount of DNA without degradation. Here we developed a new diagnostic algorithm for the accurate diagnosis of ATLL using FFPE samples. This method combines two HTL V-1 detection assays, namely, ultrasensitive RNA in situ hybridization using RNAscope for HTLV-1 bZIP factor (HBZ-RNAscope), and quantitative PCR targeting the tax gene (tax-qPCR). We analyzed 119 FFPE tissue specimens (62 ATLL, and 57 non-ATLL, including 41 HTLV-1 carriers) and compared them with the SBH results using the corresponding fresh-frozen samples. As a result, tax-qPCR had a higher ATLL identification rate than HBZ-RNAscope (88% [52/59], and 63% [39/62], respectively). However, HBZ-RNAscope clearly visualized the localization of HTLV-1-infected tumor cells and its identification rate increased to 94% (17/18) when the analysis was limited to samples up to 2 years old, indicating its usefulness in the daily diagnosis. The diagnostic algorithm combining these two assays successfully evaluated 94% (112/119) of samples and distinguished ATLL from non-ATLL cases including HTLV-1 carriers with 100% sensitivity and specificity. This method is expected to replace SBH and increase the accuracy of the diagnosis of A TLL.
言語 en
出版者
言語 ja
出版者 琉球大学
言語
言語 eng
資源タイプ
資源タイプ doctoral thesis
資源タイプ識別子 http://purl.org/coar/resource_type/c_db06
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
関連情報
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1038/s41379-020-0635-8
収録物識別子
収録物識別子タイプ PISSN
収録物識別子 0893-3952
収録物識別子タイプ EISSN
収録物識別子 1530-0285
収録物名
言語 en
収録物名 Modern Pathology
書誌情報
巻 34, p. 51-58
学位授与番号
学位授与番号 甲第547号
学位名
学位名 博士(医学)
言語 ja
学位授与年月日
学位授与年月日 2022-03-18
学位授与機関
学位授与機関識別子
学位授与機関識別子 18001
学位授与機関識別子Scheme kakenhi
学位授与機関名
学位授与機関名 琉球大学
言語 ja
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