WEKO3
アイテム
{"_buckets": {"deposit": "38f23e4b-2a0e-4c07-bde8-3f42a07e62e3"}, "_deposit": {"created_by": 8, "id": "2018043", "owner": "8", "owners": [8], "owners_ext": {"displayname": "ryukyu_lib", "username": null}, "pid": {"revision_id": 0, "type": "depid", "value": "2018043"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02018043", "sets": ["1642838159351", "1642838409905"]}, "author_link": [], "control_number": "2018043", "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "A new diagnostic algorithm using biopsy specimens in adult T-cell leukemia/lymphoma: combination of RNA in situ hybridization and quantitative PCR for HTLV-1", "subitem_1551255648112": "en"}, {"subitem_1551255647225": "成人 T 細胞白血病・リンパ腫における生検組織を用いた新規診断アルゴリズム : HTLV-1 に対する RNA in situ hybridization と定量 PCR の組み合わせ", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "髙鳥, 光徳", "creatorNameLang": "ja"}, {"creatorName": "Takatori, Mitsuyoshi", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186499011": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_1522650717957": "en", "subitem_1522651041219": "© The Author(s) 2020."}, {"subitem_1522650717957": "en", "subitem_1522650727486": "http://creativecommons.org/licenses/by/4.0/", "subitem_1522651041219": "Creative Commons Attribution 4.0 International"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "Histopathological distinction between adult T-cell leuketnia/lymphoma (ATLL) and other T-cell neoplasms is often challenging. The current gold standard for the accurate diagnosis of ATLL is the Southern blot hybridization (SBH) assay, which detects clonal integration of human T-cell leuketnia virus type I (HTLV-1) provirus. However, SBH cannot be petformed with small biopsy or formalin-fixed paraffin-embedded (FFPE) tissue samples because this assay requires a large amount of DNA without degradation. Here we developed a new diagnostic algorithm for the accurate diagnosis of ATLL using FFPE samples. This method combines two HTL V-1 detection assays, namely, ultrasensitive RNA in situ hybridization using RNAscope for HTLV-1 bZIP factor (HBZ-RNAscope), and quantitative PCR targeting the tax gene (tax-qPCR). We analyzed 119 FFPE tissue specimens (62 ATLL, and 57 non-ATLL, including 41 HTLV-1 carriers) and compared them with the SBH results using the corresponding fresh-frozen samples. As a result, tax-qPCR had a higher ATLL identification rate than HBZ-RNAscope (88% [52/59], and 63% [39/62], respectively). However, HBZ-RNAscope clearly visualized the localization of HTLV-1-infected tumor cells and its identification rate increased to 94% (17/18) when the analysis was limited to samples up to 2 years old, indicating its usefulness in the daily diagnosis. The diagnostic algorithm combining these two assays successfully evaluated 94% (112/119) of samples and distinguished ATLL from non-ATLL cases including HTLV-1 carriers with 100% sensitivity and specificity. This method is expected to replace SBH and increase the accuracy of the diagnosis of A TLL.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学"}, {"subitem_1522300295150": "en", "subitem_1522300316516": "University of the Ryukyus"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "PISSN", "subitem_1522646572813": "0893-3952"}, {"subitem_1522646500366": "EISSN", "subitem_1522646572813": "1530-0285"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "en", "subitem_1522650091861": "Modern Pathology"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicPageEnd": "58", "bibliographicPageStart": "51", "bibliographicVolumeNumber": "34"}]}, "item_1617187087799": {"attribute_name": "Dissertation Number", "attribute_value_mlt": [{"subitem_1551256171004": "甲第547号"}]}, "item_1617187112279": {"attribute_name": "Degree Name", "attribute_value_mlt": [{"subitem_1551256126428": "博士(医学)", "subitem_1551256129013": "ja"}]}, "item_1617187136212": {"attribute_name": "Date Granted", "attribute_value_mlt": [{"subitem_1551256096004": "2022-03-18"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617353299429": {"attribute_name": "Relation", "attribute_value_mlt": [{"subitem_1522306287251": {"subitem_1522306382014": "DOI", "subitem_1522306436033": "https://doi.org/10.1038/s41379-020-0635-8"}}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2022-06-15"}], "download_preview_message": "", "file_order": 0, "filename": "iken547abstract.pdf", "filesize": [{"value": "875 KB"}], "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 875000.0, "url": {"objectType": "abstract", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2018043/files/iken547abstract.pdf"}, "version_id": "d139be97-d085-4c97-8d6a-ed7cc2c169bf"}, {"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2022-06-15"}], "download_preview_message": "", "file_order": 1, "filename": "iken547review.pdf", "filesize": [{"value": "803 KB"}], "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 803000.0, "url": {"objectType": "other", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2018043/files/iken547review.pdf"}, "version_id": "983d48cb-da1e-4637-a54d-9b14cafebe21"}, {"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2022-06-15"}], "download_preview_message": "", "file_order": 2, "filename": "iken547text.pdf", "filesize": [{"value": "3.1 MB"}], "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 3100000.0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2018043/files/iken547text.pdf"}, "version_id": "65bcac1d-8d1d-48db-887e-66d58ba8bc98"}]}, "item_1617944105607": {"attribute_name": "Degree Grantor", "attribute_value_mlt": [{"subitem_1551256015892": [{"subitem_1551256027296": "18001", "subitem_1551256029891": "kakenhi"}], "subitem_1551256037922": [{"subitem_1551256042287": "琉球大学", "subitem_1551256047619": "ja"}]}]}, "item_title": "A new diagnostic algorithm using biopsy specimens in adult T-cell leukemia/lymphoma: combination of RNA in situ hybridization and quantitative PCR for HTLV-1", "item_type_id": "59", "owner": "8", "path": ["1642838159351", "1642838409905"], "permalink_uri": "http://hdl.handle.net/20.500.12000/0002018043", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-06-16"}, "publish_date": "2022-06-16", "publish_status": "0", "recid": "2018043", "relation": {}, "relation_version_is_last": true, "title": ["A new diagnostic algorithm using biopsy specimens in adult T-cell leukemia/lymphoma: combination of RNA in situ hybridization and quantitative PCR for HTLV-1"], "weko_shared_id": -1}
成人 T 細胞白血病・リンパ腫における生検組織を用いた新規診断アルゴリズム : HTLV-1 に対する RNA in situ hybridization と定量 PCR の組み合わせ
http://hdl.handle.net/20.500.12000/0002018043
http://hdl.handle.net/20.500.12000/0002018043bb2ce9b2-d6f2-4982-821d-70bd40e8c6ee
名前 / ファイル | ライセンス | アクション |
---|---|---|
iken547abstract.pdf (875 KB)
|
|
|
iken547review.pdf (803 KB)
|
|
|
iken547text.pdf (3.1 MB)
|
|
Item type | 琉球大学リポジトリ登録用アイテムタイプ(1) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2022-06-16 | |||||||||
タイトル | ||||||||||
タイトル | A new diagnostic algorithm using biopsy specimens in adult T-cell leukemia/lymphoma: combination of RNA in situ hybridization and quantitative PCR for HTLV-1 | |||||||||
言語 | en | |||||||||
タイトル | ||||||||||
タイトル | 成人 T 細胞白血病・リンパ腫における生検組織を用いた新規診断アルゴリズム : HTLV-1 に対する RNA in situ hybridization と定量 PCR の組み合わせ | |||||||||
言語 | ja | |||||||||
作成者 |
髙鳥, 光徳
× 髙鳥, 光徳
|
|||||||||
アクセス権 | ||||||||||
アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
権利情報 | ||||||||||
言語 | en | |||||||||
権利情報 | © The Author(s) 2020. | |||||||||
権利情報 | ||||||||||
言語 | en | |||||||||
権利情報Resource | http://creativecommons.org/licenses/by/4.0/ | |||||||||
権利情報 | Creative Commons Attribution 4.0 International | |||||||||
内容記述 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | Histopathological distinction between adult T-cell leuketnia/lymphoma (ATLL) and other T-cell neoplasms is often challenging. The current gold standard for the accurate diagnosis of ATLL is the Southern blot hybridization (SBH) assay, which detects clonal integration of human T-cell leuketnia virus type I (HTLV-1) provirus. However, SBH cannot be petformed with small biopsy or formalin-fixed paraffin-embedded (FFPE) tissue samples because this assay requires a large amount of DNA without degradation. Here we developed a new diagnostic algorithm for the accurate diagnosis of ATLL using FFPE samples. This method combines two HTL V-1 detection assays, namely, ultrasensitive RNA in situ hybridization using RNAscope for HTLV-1 bZIP factor (HBZ-RNAscope), and quantitative PCR targeting the tax gene (tax-qPCR). We analyzed 119 FFPE tissue specimens (62 ATLL, and 57 non-ATLL, including 41 HTLV-1 carriers) and compared them with the SBH results using the corresponding fresh-frozen samples. As a result, tax-qPCR had a higher ATLL identification rate than HBZ-RNAscope (88% [52/59], and 63% [39/62], respectively). However, HBZ-RNAscope clearly visualized the localization of HTLV-1-infected tumor cells and its identification rate increased to 94% (17/18) when the analysis was limited to samples up to 2 years old, indicating its usefulness in the daily diagnosis. The diagnostic algorithm combining these two assays successfully evaluated 94% (112/119) of samples and distinguished ATLL from non-ATLL cases including HTLV-1 carriers with 100% sensitivity and specificity. This method is expected to replace SBH and increase the accuracy of the diagnosis of A TLL. | |||||||||
言語 | en | |||||||||
出版者 | ||||||||||
言語 | ja | |||||||||
出版者 | 琉球大学 | |||||||||
出版者 | ||||||||||
言語 | en | |||||||||
出版者 | University of the Ryukyus | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ | doctoral thesis | |||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
出版タイプ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
関連情報 | ||||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | https://doi.org/10.1038/s41379-020-0635-8 | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 0893-3952 | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | EISSN | |||||||||
収録物識別子 | 1530-0285 | |||||||||
収録物名 | ||||||||||
言語 | en | |||||||||
収録物名 | Modern Pathology | |||||||||
書誌情報 |
巻 34, p. 51-58 |
|||||||||
学位授与番号 | ||||||||||
学位授与番号 | 甲第547号 | |||||||||
学位名 | ||||||||||
学位名 | 博士(医学) | |||||||||
言語 | ja | |||||||||
学位授与年月日 | ||||||||||
学位授与年月日 | 2022-03-18 | |||||||||
学位授与機関 | ||||||||||
学位授与機関識別子 | 18001 | |||||||||
学位授与機関識別子Scheme | kakenhi | |||||||||
学位授与機関名 | 琉球大学 | |||||||||
言語 | ja |