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成人 T 細胞白血病・リンパ腫における生検組織を用いた新規診断アルゴリズム ： HTLV-1 に対する RNA in situ hybridization と定量 PCR の組み合わせ
http://hdl.handle.net/20.500.12000/0002018043
http://hdl.handle.net/20.500.12000/0002018043bb2ce9b2-d6f2-4982-821d-70bd40e8c6ee
| 名前 / ファイル | ライセンス | アクション | |
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iken547abstract.pdf (875 KB)
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iken547review.pdf (803 KB)
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iken547text.pdf (3.1 MB)
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| Item type | 琉球大学リポジトリ登録用アイテムタイプ(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2022-06-16 | |||||||||
| タイトル | ||||||||||
| タイトル | 成人 T 細胞白血病・リンパ腫における生検組織を用いた新規診断アルゴリズム ： HTLV-1 に対する RNA in situ hybridization と定量 PCR の組み合わせ | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
髙鳥, 光徳
× 髙鳥, 光徳
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 権利情報 | ||||||||||
| 言語 | en | |||||||||
| 権利情報 | © The Author(s) 2020. | |||||||||
| 言語 | en | |||||||||
| 権利情報Resource | http://creativecommons.org/licenses/by/4.0/ | |||||||||
| 権利情報 | Creative Commons Attribution 4.0 International | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Histopathological distinction between adult T-cell leuketnia/lymphoma (ATLL) and other T-cell neoplasms is often challenging. The current gold standard for the accurate diagnosis of ATLL is the Southern blot hybridization (SBH) assay, which detects clonal integration of human T-cell leuketnia virus type I (HTLV-1) provirus. However, SBH cannot be petformed with small biopsy or formalin-fixed paraffin-embedded (FFPE) tissue samples because this assay requires a large amount of DNA without degradation. Here we developed a new diagnostic algorithm for the accurate diagnosis of ATLL using FFPE samples. This method combines two HTL V-1 detection assays, namely, ultrasensitive RNA in situ hybridization using RNAscope for HTLV-1 bZIP factor (HBZ-RNAscope), and quantitative PCR targeting the tax gene (tax-qPCR). We analyzed 119 FFPE tissue specimens (62 ATLL, and 57 non-ATLL, including 41 HTLV-1 carriers) and compared them with the SBH results using the corresponding fresh-frozen samples. As a result, tax-qPCR had a higher ATLL identification rate than HBZ-RNAscope (88% [52/59], and 63% [39/62], respectively). However, HBZ-RNAscope clearly visualized the localization of HTLV-1-infected tumor cells and its identification rate increased to 94% (17/18) when the analysis was limited to samples up to 2 years old, indicating its usefulness in the daily diagnosis. The diagnostic algorithm combining these two assays successfully evaluated 94% (112/119) of samples and distinguished ATLL from non-ATLL cases including HTLV-1 carriers with 100% sensitivity and specificity. This method is expected to replace SBH and increase the accuracy of the diagnosis of A TLL. | |||||||||
| 言語 | en | |||||||||
| 出版者 | ||||||||||
| 言語 | ja | |||||||||
| 出版者 | 琉球大学 | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 関連情報 | ||||||||||
| 関連識別子 | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.1038/s41379-020-0635-8 | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | PISSN | |||||||||
| 収録物識別子 | 0893-3952 | |||||||||
| 収録物識別子タイプ | EISSN | |||||||||
| 収録物識別子 | 1530-0285 | |||||||||
| 収録物名 | ||||||||||
| 言語 | en | |||||||||
| 収録物名 | Modern Pathology | |||||||||
| 書誌情報 |
巻 34, p. 51-58 |
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| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第547号 | |||||||||
| 学位名 | ||||||||||
| 学位名 | 博士(医学) | |||||||||
| 言語 | ja | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2022-03-18 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子 | ||||||||||
| 学位授与機関識別子 | 18001 | |||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関名 | ||||||||||
| 学位授与機関名 | 琉球大学 | |||||||||
| 言語 | ja | |||||||||
