Item type |
琉球大学リポジトリ登録用アイテムタイプ(1) |
公開日 |
2023-03-08 |
タイトル |
|
|
タイトル |
Comparison of Tissue Loading Before and After the Creation of a Continuous Density Gradient in Porcine Islet Purification |
|
言語 |
en |
作成者 |
Miyagi-Shiohira, Chika
Nakashima, Yoshiki
Ebi, Nana
Hamada, Eri
Tamaki, Yoshihito
Kuwae, Kazuho
Kobayashi, Naoya
Saitoh, Issei
Watanabe, Masami
Kinjo, Takao
Noguchi, Hirofumi
|
アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利情報 |
|
|
言語 |
en |
|
権利情報 |
© The Author(s) 2018 |
権利情報 |
|
|
言語 |
en |
|
権利情報Resource |
http://www.creativecommons.org/licenses/by-nc/4.0/ |
|
権利情報 |
This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
bottle purification |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
islet transplantation |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
islet isolation |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
islet purification |
主題 |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
tissue loading |
内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
The purification step is one of the most important and difficult procedures in islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from the porcine pancreas. In this study, we evaluated the impact of the timing of tissue loading on porcine islet purification using large plastic bottles. One method involved loading digested tissue after creating a continuous density gradient (tissue after gradient [TAG]). The other method involved loading digested tissue before creating a continuous density gradient (tissue before gradient [TBG]). There were no significant differences between TAG and TBG in terms of the islet yield, rates of viability and purity, score, and in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of post-transplantation normoglycemia. Our study shows the equivalency of these two methods of islet purification. |
|
言語 |
en |
出版者 |
|
|
言語 |
en |
|
出版者 |
SAGE Publishing |
言語 |
|
|
言語 |
eng |
資源タイプ |
|
|
資源タイプ |
journal article |
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
関連情報 |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1177/2155179018781343 |
収録物識別子 |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2155-1790 |
収録物名 |
|
|
言語 |
en |
|
収録物名 |
Cell Medicine |
書誌情報 |
巻 10,
発行日 2018-06-29
|