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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 29号
  1. 部局別インデックス
  2. 農学部

Corynebacterium sepedonicum の結晶 L-オルニチントランスアミナーゼ : 反応生成物の同定(農芸化学科)

http://hdl.handle.net/20.500.12000/4023
http://hdl.handle.net/20.500.12000/4023
4d5e7856-5a85-49df-8485-3d4065538c78
名前 / ファイル ライセンス アクション
KJ00000162132.pdf KJ00000162132.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル Corynebacterium sepedonicum の結晶 L-オルニチントランスアミナーゼ : 反応生成物の同定(農芸化学科)
言語 ja
作成者 安田, 正昭

× 安田, 正昭

ja 安田, 正昭

当山, 清善

× 当山, 清善

ja 当山, 清善

与那覇, 和雄

× 与那覇, 和雄

ja 与那覇, 和雄

左右田, 健次

× 左右田, 健次

ja 左右田, 健次

Yasuda, Masaaki

× Yasuda, Masaaki

en Yasuda, Masaaki

Toyama, Seizen

× Toyama, Seizen

en Toyama, Seizen

Yonaha, Kazuo

× Yonaha, Kazuo

en Yonaha, Kazuo

Soda, Kenji

× Soda, Kenji

en Soda, Kenji

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 Corynebacterium sepedonicum (IFO 3306)のL-オルニチントランスアミナーゼ(結晶標品)のL-オルニチンに対する作用特性を明らかにした。酵素反応によりL-オルニチンに由来する反応生成物は, イオン交換クロマトグラフィー及び高圧濾紙電気泳動などの分析結果から⊿^1-ピロリン-5-カルボン酸であることが確められた。一方, α-ケトグルタル酸からはL-グルタミン酸が生成された。これらのことから, 本酵素はL-オルニチンの末端アミノ基とα-ケトグルタル酸との間のアミノ基転移反応を触媒し, L-グルタミン酸とグルタミン酸-γ-セミアルデヒドを生成するが, 後者は非酵素的に脱水閉環して⊿^1-ピロリン-5-カルボン酸に変化することが明らかになった。
内容記述タイプ Other
内容記述 The reaction product of L-ornithine with crystalline L-ornithine transaminase from Corynebacterium sepedonicum (IFO 3306) was investigated. The formation of ⊿^1-pyrroline-5-carboxylate was shown by amino acid analysis and paper electrophoresis of the reaction product. These findings revealed that the terminal amino group of L-ornithine is transaminated to yield ⊿^1-pyrroline-5-carboxylate. Therefore, glutamate-γ-semialdehyde is produced directly from L-ornithine. Glutamate formed was identified as the L-enantiomer with L-glutamate dehydrogenase.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4023
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 29, p. 47-52, 発行日 1982-12-01
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