{"_buckets": {"deposit": "5936526c-e64f-466e-90c9-4495b5a62a48"}, "_deposit": {"id": "2003115", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003115"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003115", "sets": ["1642837912269", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on arginine ester hydrolyzing enzyme of Sakishima habu (T. elegans) venom (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "知念, 功", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "大城, ゆみ子", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "饒平名, とく子", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "小渡, 恵子", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "兼村, 憲勝", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "岩永, 貞昭", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Chinen, Isao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Oshiro, Yumiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nohina, Tokuko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Odo, Keiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kanemura, Norikatsu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Iwanaga, Sadaaki", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "サキシマハブ毒のタンパク質水解活性を調べた結果, Tos-Arg-OMeHCl, Casein, Insoluble Collagene, Tos-Lys-OMeHCl, Ac-L-Gly-Lys-OMe AcOHに対する水解活性が見られた。その内でもTAME活性は, 49.41(x10^\u003c-3\u003eM TAME/mg/min)で高い値であった。このTAME水解酵素は, pH2.5\u0026acd;11の範で安定であったが, 70℃以上(10分間)に加熱するとその活性は, 急激に減少した。またその酵素の至適温度は, 45℃であった。この蛇毒をセファデックスG-75カラムにのせ精製すると2個の活性ピークが見られ, その内の主要活性ピーク部をさらにセファデックスG-75,DEAEセルロースカラムを用いて精製すると, 最終的にこの分画から3個の活性ピークが見られた。", "subitem_description_type": "Other"}, {"subitem_description": "Sakishima habu venom hydrolyzes Tos-Arg-OMeHCl (49.41 TAME×10^\u003c-3\u003eM/mg/min), casein (13.26 tyrosine μg/mg/min), insoluble collagen (3.26 hypro. μg/mg/min), Tos-Lys-OMeHCl (1.43 TLME×10^\u003c-3\u003eM/mg/min), and Ac-L-Gly-Lys-OMeOH (0.38 AGLE×10^\u003c-3\u003eM/mg/min). The TAME hydrolyzing enzyme is stable in a pH region of 2.5 to 11 and at temperature below 50℃ (10 min); however it becomes unstable over 70℃. The enzyme activity was maxium at 45℃. Sephadex G-75 gel filtration of the venom was found to contain two active peaks, and further purification of the main active fraction by Sephadex G-75 and DEAE cellulose column chromatography was demonstrated to have three active peaks.", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4086"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1980-11-29", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "27", "bibliographicPageEnd": "68", "bibliographicPageStart": "57"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162044.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003115/files/KJ00000162044.pdf"}, "version_id": "3b0cec5e-7964-4525-9257-a884ba635389"}]}, "item_title": "サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)", "item_type_id": "15", "owner": "1", "path": ["1642837912269", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4086", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003115", "relation": {}, "relation_version_is_last": true, "title": ["サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)"], "weko_shared_id": -1}
サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)
http://hdl.handle.net/20.500.12000/4086
http://hdl.handle.net/20.500.12000/4086