{"_buckets": {"deposit": "5936526c-e64f-466e-90c9-4495b5a62a48"}, "_deposit": {"id": "2003115", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003115"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003115", "sets": ["1642837912269", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u30b5\u30ad\u30b7\u30de\u30cf\u30d6\u6bd2\u306e\u30a2\u30eb\u30ae\u30cb\u30f3\u30a8\u30b9\u30c6\u30eb\u6c34\u89e3\u9175\u7d20\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on arginine ester hydrolyzing enzyme of Sakishima habu (T. elegans) venom (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u77e5\u5ff5, \u529f", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5927\u57ce, \u3086\u307f\u5b50", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u9952\u5e73\u540d, \u3068\u304f\u5b50", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5c0f\u6e21, \u6075\u5b50", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u517c\u6751, \u61b2\u52dd", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5ca9\u6c38, \u8c9e\u662d", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Chinen, Isao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Oshiro, Yumiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nohina, Tokuko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Odo, Keiko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Kanemura, Norikatsu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Iwanaga, Sadaaki", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u30b5\u30ad\u30b7\u30de\u30cf\u30d6\u6bd2\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u6c34\u89e3\u6d3b\u6027\u3092\u8abf\u3079\u305f\u7d50\u679c, Tos-Arg-OMeHCl, Casein, Insoluble Collagene, Tos-Lys-OMeHCl, Ac-L-Gly-Lys-OMe AcOH\u306b\u5bfe\u3059\u308b\u6c34\u89e3\u6d3b\u6027\u304c\u898b\u3089\u308c\u305f\u3002\u305d\u306e\u5185\u3067\u3082TAME\u6d3b\u6027\u306f, 49.41(x10^\u003c-3\u003eM TAME/mg/min)\u3067\u9ad8\u3044\u5024\u3067\u3042\u3063\u305f\u3002\u3053\u306eTAME\u6c34\u89e3\u9175\u7d20\u306f, pH2.5\u0026acd;11\u306e\u7bc4\u3067\u5b89\u5b9a\u3067\u3042\u3063\u305f\u304c, 70\u2103\u4ee5\u4e0a(10\u5206\u9593)\u306b\u52a0\u71b1\u3059\u308b\u3068\u305d\u306e\u6d3b\u6027\u306f, \u6025\u6fc0\u306b\u6e1b\u5c11\u3057\u305f\u3002\u307e\u305f\u305d\u306e\u9175\u7d20\u306e\u81f3\u9069\u6e29\u5ea6\u306f, 45\u2103\u3067\u3042\u3063\u305f\u3002\u3053\u306e\u86c7\u6bd2\u3092\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9G-75\u30ab\u30e9\u30e0\u306b\u306e\u305b\u7cbe\u88fd\u3059\u308b\u30682\u500b\u306e\u6d3b\u6027\u30d4\u30fc\u30af\u304c\u898b\u3089\u308c, \u305d\u306e\u5185\u306e\u4e3b\u8981\u6d3b\u6027\u30d4\u30fc\u30af\u90e8\u3092\u3055\u3089\u306b\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9G-75,DEAE\u30bb\u30eb\u30ed\u30fc\u30b9\u30ab\u30e9\u30e0\u3092\u7528\u3044\u3066\u7cbe\u88fd\u3059\u308b\u3068, \u6700\u7d42\u7684\u306b\u3053\u306e\u5206\u753b\u304b\u30893\u500b\u306e\u6d3b\u6027\u30d4\u30fc\u30af\u304c\u898b\u3089\u308c\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "Sakishima habu venom hydrolyzes Tos-Arg-OMeHCl (49.41 TAME\u00d710^\u003c-3\u003eM/mg/min), casein (13.26 tyrosine \u03bcg/mg/min), insoluble collagen (3.26 hypro. \u03bcg/mg/min), Tos-Lys-OMeHCl (1.43 TLME\u00d710^\u003c-3\u003eM/mg/min), and Ac-L-Gly-Lys-OMeOH (0.38 AGLE\u00d710^\u003c-3\u003eM/mg/min). The TAME hydrolyzing enzyme is stable in a pH region of 2.5 to 11 and at temperature below 50\u2103 (10 min); however it becomes unstable over 70\u2103. The enzyme activity was maxium at 45\u2103. Sephadex G-75 gel filtration of the venom was found to contain two active peaks, and further purification of the main active fraction by Sephadex G-75 and DEAE cellulose column chromatography was demonstrated to have three active peaks.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4086"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "27", "bibliographicPageEnd": "68", "bibliographicPageStart": "57"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162044.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003115/files/KJ00000162044.pdf"}, "version_id": "3b0cec5e-7964-4525-9257-a884ba635389"}]}, "item_title": "\u30b5\u30ad\u30b7\u30de\u30cf\u30d6\u6bd2\u306e\u30a2\u30eb\u30ae\u30cb\u30f3\u30a8\u30b9\u30c6\u30eb\u6c34\u89e3\u9175\u7d20\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837912269", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4086", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003115", "relation": {}, "relation_version_is_last": true, "title": ["\u30b5\u30ad\u30b7\u30de\u30cf\u30d6\u6bd2\u306e\u30a2\u30eb\u30ae\u30cb\u30f3\u30a8\u30b9\u30c6\u30eb\u6c34\u89e3\u9175\u7d20\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}