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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 27号
  1. 部局別インデックス
  2. 農学部

サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)

http://hdl.handle.net/20.500.12000/4086
http://hdl.handle.net/20.500.12000/4086
f6fca30c-f197-483e-9933-5f3b2da140ce
名前 / ファイル ライセンス アクション
KJ00000162044.pdf KJ00000162044.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)
言語 ja
作成者 知念, 功

× 知念, 功

ja 知念, 功

大城, ゆみ子

× 大城, ゆみ子

ja 大城, ゆみ子

饒平名, とく子

× 饒平名, とく子

ja 饒平名, とく子

小渡, 恵子

× 小渡, 恵子

ja 小渡, 恵子

兼村, 憲勝

× 兼村, 憲勝

ja 兼村, 憲勝

岩永, 貞昭

× 岩永, 貞昭

ja 岩永, 貞昭

Chinen, Isao

× Chinen, Isao

en Chinen, Isao

Oshiro, Yumiko

× Oshiro, Yumiko

en Oshiro, Yumiko

Nohina, Tokuko

× Nohina, Tokuko

en Nohina, Tokuko

Odo, Keiko

× Odo, Keiko

en Odo, Keiko

Kanemura, Norikatsu

× Kanemura, Norikatsu

en Kanemura, Norikatsu

Iwanaga, Sadaaki

× Iwanaga, Sadaaki

en Iwanaga, Sadaaki

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 サキシマハブ毒のタンパク質水解活性を調べた結果, Tos-Arg-OMeHCl, Casein, Insoluble Collagene, Tos-Lys-OMeHCl, Ac-L-Gly-Lys-OMe AcOHに対する水解活性が見られた。その内でもTAME活性は, 49.41(x10^<-3>M TAME/mg/min)で高い値であった。このTAME水解酵素は, pH2.5&acd;11の範で安定であったが, 70℃以上(10分間)に加熱するとその活性は, 急激に減少した。またその酵素の至適温度は, 45℃であった。この蛇毒をセファデックスG-75カラムにのせ精製すると2個の活性ピークが見られ, その内の主要活性ピーク部をさらにセファデックスG-75,DEAEセルロースカラムを用いて精製すると, 最終的にこの分画から3個の活性ピークが見られた。
内容記述タイプ Other
内容記述 Sakishima habu venom hydrolyzes Tos-Arg-OMeHCl (49.41 TAME×10^<-3>M/mg/min), casein (13.26 tyrosine μg/mg/min), insoluble collagen (3.26 hypro. μg/mg/min), Tos-Lys-OMeHCl (1.43 TLME×10^<-3>M/mg/min), and Ac-L-Gly-Lys-OMeOH (0.38 AGLE×10^<-3>M/mg/min). The TAME hydrolyzing enzyme is stable in a pH region of 2.5 to 11 and at temperature below 50℃ (10 min); however it becomes unstable over 70℃. The enzyme activity was maxium at 45℃. Sephadex G-75 gel filtration of the venom was found to contain two active peaks, and further purification of the main active fraction by Sephadex G-75 and DEAE cellulose column chromatography was demonstrated to have three active peaks.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4086
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 27, p. 57-68
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