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[原著]Specific and Sensitive Estradiol-17 $ \beta $ Radioimmunoassay
http://hdl.handle.net/20.500.12000/0002015892
http://hdl.handle.net/20.500.12000/00020158926d39ef58-75d2-451b-8b53-b9ceccbe5c02
名前 / ファイル | ライセンス | アクション |
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v12p219.pdf
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Item type | デフォルトアイテムタイプ(フル)(1) | |||||||||
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公開日 | 2010-07-02 | |||||||||
タイトル | ||||||||||
タイトル | [原著]Specific and Sensitive Estradiol-17 $ \beta $ Radioimmunoassay | |||||||||
言語 | ja | |||||||||
作成者 |
Kono, Shinzo
× Kono, Shinzo
× Sunagawa, Hajime
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アクセス権 | ||||||||||
アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
権利情報 | ||||||||||
言語 | ja | |||||||||
権利情報 | 琉球医学会 | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | radioimmunoassay | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | estradiol-17 $ \beta $ | |||||||||
主題 | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | postmenopausal women | |||||||||
内容記述 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | A specific and highly sensitive radioimmunoassay of estradiol-17$ \beta $ (E_2-17$ \beta $) was developed. Ether was used to extract E_2-I7 from 0.5ml plasma samples and the extract was then purified by Sephadex LH-20 column chromatography and measured using an anti-serum to E_2-6-BSA and I^<125>-labelled E_2-17$ \beta $ Sensitivity of the method was 2.5pg/ml. The coefficient of variation in accuracy and precision of the method was under 15%. The unconjugated E_2-17$ \beta $ concentration in sera of 21 postmenopausal women ranged from 2.5 to 14.1 pg/ml. In serum samples over 10 pg/ml investigated by the direct method, E_2-17$ \beta $ values determined by the present method correlated well with the direct method (r=0.91,pく0.001, N=87). In samples under 10 pg/ml determined by the direct method, E_2-17$ \beta $ value ranged from 2.5 to 23.5 pg/ml by the present method. The present method might be useful for more accurate evaluations of ovarian functions. | |||||||||
内容記述 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | 論文 | |||||||||
出版者 | ||||||||||
言語 | ja | |||||||||
出版者 | 琉球医学会 | |||||||||
出版者 | ||||||||||
言語 | en | |||||||||
出版者 | Ryukyu Medical Association | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ | journal article | |||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
出版タイプ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 1346888X | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 0289-1530 | |||||||||
収録物識別子 | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AN10369445 | |||||||||
収録物名 | ||||||||||
言語 | ja | |||||||||
収録物名 | 琉球医学会誌 = Ryukyu Medical Journal | |||||||||
書誌情報 |
巻 12, 号 3, p. 219-225, 発行日 1992 |